Store the jars in a clean place (in front of a HEPA-filter or in a glove box). Wash and disinfect your hands and forearms and use latex gloves. Open the lid as soon as the pressure has reached zero. To keep contamination risk low, please work clean! Use a new, sterile scalpel blade for each spore print. You can put a clean wet piece of cloth over the cooker during the cooling process to filter the air streaming into the cooker, if working without a HEPA-filter. Leave the cooker to cool out in clean place or in front of HEPA-filter after sterilization.
SPORE SYRINGE PROFESSIONAL
If you have a professional pressure steriliser at your disposal, sterilise at 121☌/ 250☏/ 15 psi/ 1.05 bar. We recommend using a pressure cooker for sterilisation.Ĭounting the sterilisation time from the moment, the pressure gauge reaches its highest stage (for household pressure cookers). Wrap aluminium foil over and around the lid, sterilise the glasses and the lid with a hole AND one additional lid without a hole (that one will be used for mixing the water with the spores - also wrap this one in aluminium foil), for about 30 minutes. Fill the jars with water (2/3 of the jar) and put on the lid with the hole during sterilisation. The lid of the jars needs a hole of about 1 cm in diameter via this hole you will later fill the syringes. We recommend using about 100 ml water and one spore print per jar. If you inoculate substrates with a spore syringe, you get a “Multi-culture” (look up “Growing mycelium out of spores”), which mostly results in less crop yield, compared to the use of selected rhizomorph mycelium strains. It’s rather easy to produce small to medium amounts of substrates or grain spawn with spore syringes. Hobby-mycologists tend to work with spore syringes, because even if used in a not 100 % sterile environment, the substrates are seldom contaminated. Spore syringes contain spores dissolved in water and are used to inoculate substrates. King stropharia - Stropharia rugosoannulata.Brown-Gilled woodlover - Hypholoma capnoides.Umbrella Polypore - Polyporus Umbellatus.Caterpillar fungus - Cordyceps sinensis.Winter mushroom (Enokitake) - Flammulina velutipes.Almond mushroom - Agaricus blazei murril.Beech mushroom - Hypsizygus tessellatus.Golden Oyster - Pleurotus citrinopileatus.Pink Oyster Mushroom - Pleurotus salmoneostramineus.
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